A qualitative study of pregnant women’s perceptions and decision-making regarding COVID-19 vaccination in Thailand

To identify pregnant women’s attitudes towards, and acceptance and rejection of, COVID-19 vaccination. This prospective, descriptive, implementation study was conducted in the Antenatal clinic of Siriraj Hospital, Bangkok, Thailand. In Phase I, 40 pregnant women were interviewed. Phase II consisted of questionnaire development and data validation. In Phase III, the questionnaire was administered to 400 participants. Pregnant women’s attitudes towards and acceptance and rejection of COVID-19 vaccination. Most pregnant women were uncertain about the potential harm of vaccination to themselves or their unborn child, including risks such as miscarriage or premature birth (59–66/101 [58.4%–65.3%]; OR 2.53–8.33; 95% CI 1.23–3.60, 5.17–19.30; P < 0.001) compared to those who disagreed with vaccination. Their vaccination decisions were significantly influenced by social media information regarding vaccination complications in pregnant women (74/101 [73.3%]; OR 15.95; 95% CI 2.15–118.55; P = 0.001) compared to those who disagreed with vaccination. Most pregnant women opined that they should not receive a COVID-19 vaccination during pregnancy (adjusted odds ratio [AOR] 6.57; 95% CI 2.44–17.70; P = 0.001). Most also rejected vaccination despite being aware of its benefits (AOR 17.14; 95% CI 6.89–42.62; P < 0.001). Social media messages and obligatory vaccination certifications influence maternal vaccination decisions. Pregnant women believe vaccination helps prevent COVID-19 infection and reduces its severity. Nevertheless, the primary reason for their refusal was concern about potential harm to their unborn child or themselves during pregnancy. The Thai clinical trials registry: TCTR20211126006.


Materials and methods
This was a prospective, descriptive study.The Siriraj Ethics Committee of the Faculty of Medicine at Siriraj Hospital approved the protocol (Si1018/2021).The Thai Clinical Trials registration number is TCTR20211126006.
This survey study utilised validated questionnaires (Phase III).The sample size was calculated using a proportion of the results of interest of 50% (P = 0.5), an estimation error of ≤ 5% and a 95% confidence level (type I error = 0.05; 2-sided).The number of pregnant women who needed to be surveyed was ≥ 385.

Phase I: in-depth interviews
To find participants for Phases I and III, pregnant women of any gestational age attending the hospital's antenatal ward without any restriction were invited to a private counselling room where the research project was described.The pregnant women participating in the study were Thai women aged at least 16 years and literate.The women were given time to ask questions and consider whether they wished to enrol in the trial.They were advised that they could decline to participate or withdraw at any stage and for any reason if they did enrol.All women who volunteered to be research subjects for Phases I and III were asked to sign an informed consent form.
This phase collected information about the following 4 areas: 1. General pregnant women information 2. Pregnant women attitudes towards COVID-19 vaccination, knowledge of complications of COVID-19 infection during pregnancy, and reasons for accepting or rejecting COVID-19 vaccination 3. Decision-making about COVID-19 vaccination www.nature.com/scientificreports/ 4. Frustrations with deciding whether to be vaccinated We conducted in-depth interviews with pregnant women to explore their knowledge, attitudes, acceptance, and refusal of vaccination.The insights gathered from these interviews were then utilized to develop a questionnaire.
Between 1st May and 15nd June 2022, in-depth interviews for Phase I were conducted with 40 women.Before these interviews commenced, the participants were asked for permission for the conversations and their structured interview to be audio-recorded.Forty-five pregnant women were invited to participate in the in-depth interview phase.Five pregnant women declined the interview because they were uncomfortable while answering the question and being audio-recorded.The subjects initially completed an attitude assessment questionnaire.It dealt with their attitudes, knowledge of the complications of a COVID-19 infection during pregnancy, and their reasons for accepting or rejecting COVID-19 vaccination.Several other aspects were then investigated in a structured interview.One aspect related to their decision-making for COVID-19 vaccination and any frustration they felt before deciding to be vaccinated.
The total time from the commencement of the questionnaire until the completion of the comprehensive interview was approximately 30 min.The data integrity of the research questions was later verified.The completeness of data provided by patients in the basic demographic questions regarding their history of vaccination, type of vaccination, and acceptance or rejection was reexamined to ensure data integrity.

Phase II: questionnaire development and validation
Between 16th June and 31st July 2022, the data from the provisional questionnaire (non-revised and non-validated questionnaire) and the in-depth interviews administered during Phase I were analysed to determine the means and standard deviations.Doing so enabled the questionnaire and the interview questions to be refined.The validity and reliability of the revised questionnaire and interview questions were tested before their use in the next phase.The revised questionnaire and interview questions underwent testing for validity and reliability before their implementation in the subsequent phase.A specialized statistician assessed the questionnaire's validity, focusing on double-barreled, confusing, and leading questions.To evaluate the test-retest reliability, the same respondents completed the questionnaire again one month after the initial completion.Forty pregnant women were given a revised questionnaire to test its validity and accuracy.

Phase III: questionnaire administration (final phase)
Between 1st August and 31st October 2022, the validated questionnaires which has been tested for validity and accuracy, were given to 430 pregnant women (different group from Phase I) in the hospital's antenatal ward during the final phase.Out of 430 pregnant women approached for the study, 30 declined to participate, resulting in a total recruitment of 400 women in this specific timeframe.There was no dropout of pregnant women during response to questionnaire.

Statistical analysis
Descriptive statistics were used to summarise demographic data.Categorical data are presented as numbers and percentages.Continuous data are presented as mean ± standard deviation or median and range.All analyses were performed using PASW Statistics for Windows (version 18.0; SPSS Inc., Chicago, IL, USA).Multivariate analysis was performed using the forward stepwise and multiple logistic regression methods.
Univariate and multivariate logistic regression analysis were used to identify statistically significant independent factors association.Factors having a p-value of less than 0.10 in the univariate analysis were imported to multivariate logistic regression to control the effect of possible confounding factors.Factors were chosen using the forward-stepwise likelihood ratio approach in the multiple logistic regression model, and the adjusted odds ratio (AOR) with its 95% CI at a p-value of ≤ 0.05 was used to determine statistically significant association.

Institutional review board statement
The research was approved by the Ethics Committee of the Siriraj Institutional Review Board (COA [Si1018/2021]).All procedures performed in studies are not involving human participants.

Informed consent statement
Written, informed consent was obtained from all participants.
More than 50% of the respondents agreed that COVID-19 is a potentially dangerous disease and can cause miscarriages and premature births.However, over 50% were unsure whether the vaccine could protect against COVID-19, might harm an unborn baby, or could increase the risk of miscarriage or preterm birth (Fig. 1).More than half (55.8%) were confident that the COVID-19 vaccine could reduce the severity of the disease.
Of those who accepted the COVID-19 vaccination, more than 50% believed that the vaccine would protect against COVID-19, 65.6% were unsure whether the vaccine would harm their child, and 59.2% were still unsure and needed to consult others before getting vaccinated.Of those who refused to be vaccinated against COVID-19, more than 50% were unsure if the vaccine could cause foetal abnormalities or increase the risk of miscarriage or preterm birth (Fig. 2).
More than 70% of the pregnant women indicated that the core information that influenced their vaccination decisions was the following: 1. the severity of COVID-19 illness 2. the reduction in infection and disease severity resulting from vaccination 3. the potential harm to an unborn baby caused by COVID-19 vaccines 4. the level of immunity to COVID-19 in women who have been previously vaccinated (Table 2) More than 50% of the women reported that obligatory vaccination certifications impacted their daily work and their decisions to be vaccinated.Furthermore, over 50% indicated that social media information about the

Discussion
We engaged in in-depth interviews with pregnant women to delve into their knowledge, attitudes, acceptance, and refusal of vaccination.The valuable insights obtained from these interviews were instrumental in shaping the authentic data used to construct a questionnaire.
In our study, 85.3% of the pregnant women were vaccinated before becoming pregnant and understood the potential severity of COVID-19.Nevertheless, once pregnant, 50% of this subgroup had no confidence in the vaccination.They were concerned about the dangers of the vaccine to themselves and their unborn children, especially miscarriage and premature birth.This concern was evident despite their being aware that the vaccine can reduce the severity of the disease.
Our research also found that the pregnant women's level of immunity to COVID-19 did not affect their vaccination decisions.The effectiveness of vaccines varies depending on the vaccine type and evolves over time within the pregnant population.The variations in immunity can be influenced by factors such as maternal age and underlying diseases, body mass index, and gestational age 24 .Nevertheless, pregnant women continue to express concerns about their immunity following previous injections and are hesitant to receive any further vaccinations during pregnancy.
Even if they knew their immunity level, they still decided not to get vaccinated because they were concerned about possible harm to the unborn baby, miscarriage or preterm delivery.This attitude must be adjusted during the COVID-19 pandemic.Several studies have shown that vaccination against COVID-19 before and during pregnancy is safe, effective and beneficial to both the mother and child.The benefits of getting a COVID-19 vaccination during pregnancy far outweigh any potential adverse consequences 23,[25][26][27][28] .
No COVID-19 vaccine contains a live virus, so the vaccines do not cause COVID-19 infection in recipients, including pregnant women and their foetuses 23,[25][26][27][28] .However, our investigation found that most respondents www.nature.com/scientificreports/were uncertain whether the vaccine was safe for themselves and their unborn children.The women were unsure whether the vaccine would help prevent infection in their unborn babies.Most also believed that multiple vaccinations would harm their unborn children.This lack of information made it very challenging for them to decide whether to be vaccinated while pregnant.
Regarding the safety of mRNA COVID-19 vaccines (Moderna and Pfizer-BioNTech), no problems have been found for women vaccinated with them before or during pregnancy or for their unborn children 23,[25][26][27][28] .Data from studies in the United States, Europe and Canada show that their use during pregnancy is not associated with an increased risk of complications, such as preterm birth, miscarriage and postpartum haemorrhage 21,26,29 .There is no increased risk of miscarriage in pregnant women administered an mRNA COVID-19 vaccine before or during early pregnancy (before 20 gestational weeks) 25,26,28,29 .A study from Chicago found that COVID-19 www.nature.com/scientificreports/vaccination in pregnant women before and during the first trimester was not associated with a risk of congenital malformations 30 .The administration of 2 primary doses of a COVID-19 mRNA vaccine to mothers during their pregnancy helped protect babies younger than 6 months from being hospitalised due to COVID-19 infection.In our investigation, the majority (84%) of infants hospitalised with an infection were born to women not vaccinated during pregnancy 31 .
Our research found that the type and number of vaccinations influenced vaccination decisions.In Thailand, the Pfizer-BioNTech and Moderna COVID-19 vaccines are more popular than the other COVID-19 vaccines available in the country, and these 2 vaccines have been reported to be safe in pregnant women 23 .However, some  www.nature.com/scientificreports/vaccination centres in Thailand only provide 1 type of vaccine.Consequently, people seeking vaccination may find that their preferred vaccine is unavailable.If an alternative vaccine can be provided by allowing pregnant women to select the vaccine themselves, it would likely increase the vaccination rate among pregnant women.In addition, our research found that vaccination decisions are influenced by social media news about the dangers to mothers and unborn children, including death and disability.Most of the pregnant women in our study rejected vaccination because they were uncertain whether vaccination would increase their foetuses' immunity.Recent research has revealed the role of social media in disseminating information and potentially influencing people's attitudes towards vaccination.Studies have also shown the positive potential of social media in public health interventions and overcoming vaccination hesitancy among mothers [32][33][34][35] .Therefore, there should be thorough scrutiny of the various roles of social media in disseminating information to the public and influencing individual behaviour in the context of public health activities.This approach will give pregnant women a correct understanding of COVID-19 vaccines.
Vaccination certifications also play a key role in pregnant women's vaccination decisions.Attending workplaces or meetings involving large groups of people puts individuals at risk of contracting COVID-19.Therefore, most public and private organisations require employees attending workplaces to be vaccinated to the levels recommended by the Thai Ministry of Health.Employers may also require certification of COVID-19 vaccination status.These restrictive policies pressurise pregnant women to get vaccinated even if they disagree with having a vaccination.
WHO has commented that COVID-19 is a health emergency that does not give governments many choices in quickly returning the situation to normal.Regarding calls for the widespread use of COVID-19 vaccination certificates, WHO recognises that introducing such certificates is risky and may result in harm.The general use of the certificates may cause deviations from their initial objectives: to ensure continuity of care and to provide proof of vaccination status.Legal or ethical considerations may be raised by further potential uses for vaccination certificates, for example, public health surveillance, pharmacovigilance, research, and exemptions to public health and social measures.WHO cites legal obligations to protect patient data and the need to respect human rights and fundamental freedoms.To this end, WHO has recommended that data protection measures be in place before adopting digital vaccination certificates.It has also stressed that vaccination certificates must not be considered a substitute for health surveillance 36 .
Our study presented both similar and different results from the previous study about attitudes, acceptance and rejection of COVID-19 vaccination among breastfeeding women 34 .Both pregnant and breastfeeding women believed that vaccines can reduce infection and disease severity.The women's COVID-19 immunity levels did not affect their acceptance or rejection of vaccination and some mothers rejected vaccination because of concerns about possible harm to them or their newborns.The safety of COVID-19 vaccination to the unborn and newborn babies and mothers is the main concerning of both pregnant and breastfeeding women.However the different results of pregnant women from breastfeeding women were the effect of social media messages and vaccination certifications to their decision.Pregnant women had more concern about those issue than breastfeeding women.Most of pregnant women were still working and COVID-19 vaccination certifications were important to their works.While breastfeeding women have a right for stop working up to 90 days, therefore vaccination certification is not required.
To enhance COVID-19 vaccination rates during pregnancy, it is essential to address the significant decline in pregnant women's confidence in vaccination.Targeted strategies involve implementing comprehensive education and communication campaigns to dispel misinformation and underscore the safety and benefits of vaccination for both mothers and unborn children.Specific measures include developing focused educational initiatives, employing communication strategies to counter social media influence, improving information accessibility about vaccine types, establishing clear certification guidelines for safety, and tailoring messaging to address concerns about potential harm to unborn babies.These efforts aim to increase vaccination acceptance among pregnant women, contributing to improved maternal and fetal health outcomes.
Our study is affected by some limitation.The study design was prospective cross-sectional study which represented the real situation of COVID-19 outbreak during that time.The study is limited by the exclusive recruitment of the sample from Siriraj Hospital.Despite this, it's crucial to recognize that Siriraj Hospital, functioning as both a medical school and a referral center in Bangkok, draws patients from diverse regions of Thailand seeking advanced prenatal care.The selection of 400 pregnant women aimed to represent a varied demographic from different parts of the country.Although participants were not randomly chosen and were exclusively from Siriraj Hospital, the study intended to capture the extensive demographics and geographic diversity inherent in the hospital's patient population.Most of pregnant women (85.3%) had a history of COVID-19 vaccination which would affect the decision making for repeated vaccination.Their actual attitude may be affected by the severity of disease and availability of database of COVID-19 vaccination during pregnancy at that time.
The strength of our study is the less of "socially desirable" bias.In phase I, participant was in-depth interviewed in a close area by only single interviewer and in phase III, pregnant women response questionnaire in a closed place.The respondent can present the actual attitude, acceptance or rejection of COVID-19 vaccination.

Conclusions
Although COVID-19 severity has decreased, it is essential to note that this factor does not significantly influence pregnant women's decisions to accept or refuse vaccination.The study findings indicate that pregnant women hold the belief that vaccines play a crucial role in preventing infection and mitigating the severity of the disease.Nevertheless, their main reason for refusing injections is concern about potential harm to their unborn children and themselves during pregnancy.Social media communications and obligatory vaccination certifications influence maternal vaccination decisions.Providing accurate information through social media will enable pregnant

Vaccinations should not be given to pregnant women at all because of the harmful effects You should have the opportunity to choose the type of vaccine to be given The type of vaccine affects your vaccination decision Vaccines can cause a foetus to be born prematurely Vaccines cause the miscarriage of the unborn baby Vaccines cause disabilities or abnormalities in the unborn baby Vaccines cannot prevent pregnant women becoming infected with COVID- 19 Vaccines 8 )Figure 2 .
Figure 2. Patient attitude and acceptance and rejection of COVID-19 vaccination.

Table 1 .
Demographic data of pregnant women responding to questionnaire.

Table 2 .
Information for making decisions about COVID-19 vaccination (can select more than one choice).If you have been vaccinated before pregnancy, you are confident that there will be no need for further vaccinations when you are pregnant Vol:.(1234567890) Scientific Reports | (2024) 14:5128 | https://doi.org/10.1038/s41598-024-55867-z

Table 3 .
Grievances about the decision to get a vaccination.

Table 4 .
Univariable analysis of factors associated with vaccination rejection (only factors that are likely to have a relationship of P < 0.10).

Table 5 .
Multivariable analysis (using the forward stepwise method: multiple logistic regression of factors associated with rejected vaccination (only factors that are likely to have a relationship P < 0.10). https://doi.org/10.1038/s41598-024-55867-z